Seminar from Marquette University
				Hear Our Guest Speaker: Dr. Daniel Sem, Marquette University
Protasis is committed to supporting life science researchers with breakthrough new technology.
e-Seminar at:  Protasis MRM				Host:  Tim Peck
101 Tomaras Avenue Savoy, IL 217-351-4359				When:  July 15, 2008
				From:  10:00 - 11:30 AM EDT
Click here for directions to Kalypsys, Inc.
Protasis Corporation is pleased to present an e-seminar on Tuesday, July 15th from 10:00AM to 11:30AM from Protasis MRM in Illinois.  Join us via conference call.  Open to anyone interested in learning about the latest trends in MicroFlow NMR.
Integrating MicroFlow NMR into Fragment-based Drug Discovery
Space is not limited (it is a conference call), but please register now for the event at:  http://www.protasis.com/seminar/WhatsNew2008/ To learn more visit:  http://www.protasis.com/OneMinuteNMRPresentation/
See Presentations By:   Dr. Dean Olson, Director of Applications, Protasis Corporation Title: ENC2008 Highlights:  Prep Your Sample in a Convenient, Sealed Vial Protasis brought back its live demonstration of One-Minute NMR again to this year's ENC.  This year we featured our new point-of-use sample filtration vials.  Each visitor to the suite received their own filter vial and was invited to load a sample, log it in and see the results from One-Minute NMR (simulated).  Attend the highlights seminar and ask for your copy our ENC poster, featuring an integrated approach to LC-MS-NMR.  See all of the action with explanation from Dr. Olson.   Dr. Daniel Sem, Assistant Professor and Director of the CPFM, Marquette University   Title:  Integrating MicroFlow NMR into Fragment-based Drug Discovery At the CPFM (Chemical Proteomics Facility at Marquette) we are pursuing fragment-based drug discovery using protein NMR. The facility has two spectrometers: Varian 600 MHz NMR spectrometer with cryogenic probe (1H/2H/{13C}/{15N} detection, z-gradient, variable temp. control, 5mm tube sample format) that employs manual loading of sample tubes, and Varian 400 MHz NMR spectrometer equipped with a Protasis CapNMR Microflowprobe (TXI triple resonance, 1H/2H/{13C}/{15N} detection, z-gradient, variable temp. control, and 10 mL NMR flowcell) that employs automated sample introduction using a LEAP Technologies (CTC Analytics) liquid handler robot and Protasis One-Minute NMR (OMNMR) software. The CPFM, and associated drug discovery infrastructure, was designed based on lessons gained from previous affiliation with and cofounding of the first NMR-centric rational drug design company (Triad Therapeutics, Inc.) in the late 1990s in southern California, USA.  Currently, we use Microflow NMR for a number of applications, which include: Initial quality control on 15N-labeled proteins (to be used in chemical shift perturbation assays), Quality-control on chemical compound collections (especially 1D 1H and 2D COSY), and Fragment-based screening using STD (saturation transfer difference) NMR. The latter is of greatest interest, and applications will be described using an infectious disease drug target (a 120 kDa tetrameric protein). Specifically, we have developed an assay strategy using lower affinity reporter ligands to identify fragments to be used in a fragment assembly drug discovery effort. Complementary use of this fragment assembly strategy with thiol tethering will also be discussed. Robert Albrecht, Director of Automation, Protasis Corporation Title: One-Minute NMR: Automated NMR for the Serious Medicinal Chemist Protasis software already provides convenient multi-user, web-based sample log-in, fast sample loading, complete HT-NMR system monitoring and network connectivity to structure confirmation packages from a simple, web-based graphical user interface that readily imports a wide array of data formats and file architectures.  The leading product in NMR automation continues to expand its scope with the integration of front-end importing of sample lists from purification stations (e.g., HPLC, SPE) and back-end data processing software and molecular structure confirmation. One-Minute NMR delivers the easiest operator interface and most flexible sample formats of any commercially available NMR automation system. The rich feature set, arrived at through our collaborations with real customers, is discussed in detail along with cutting-edge new data processing features that re-define the standard for integrated NMR. Whether your scientists use NMR for structure confirmation, elucidation, or even quantitation, you will want to learn how to get all the information you need with minimum effort and cost.   Please register at: http://www.protasis.com/seminar/WhatsNew2007/   Contact David Strand at 508-481-4163 or by email at d.strand@protasis for questions or additional details