See New Data Sets in LC-MS-NMR!
				*Hear Our Guest Speaker:* Roger Kautz, Northeastern University, Boston, MA
Protasis is committed to supporting life science researchers with breakthrough new technology.

e-Seminar at: Protasis MRM / UIUC				Host: Tim Peck
101 Tomaras Avenue Savoy, IL 61874				When: March 6, 2007
				From: 3:30-5:00PM EST

Protasis Corporation is pleased to present an e-seminar on Tuesday, March 6th from 3:30PM to 5:00PM EST from its offices at 101 Tomaras Ave., Savoy, IL via conference call for anyone interested in learning about the latest trends in MicroFlow NMR.

*Toward Optimized LC-MS+NMR Strategies* *with CapNMR Probes*

Please register now for the event at: http://www.protasis.com/seminar/WhatsNew2007/ We will send instructions to download the slides and join the conference call on the day of the event. To learn more visit: http://www.protasis.com/OneMinuteNMRPresentation/ To See our Extended Year-End Special Offers, go to: http://www.protasis.com/Offers/


See Presentations By: Tim Peck, President, MRM Division, Protasis Corporation NMR as Part of a Healthier Future: A Report on Metabonomics Day at U-Buffalo See what went on at the Metabonomics Day organized by Thomas Szyperski at SUNY-Buffalo on January 8th featuring the huge potential for NMR in biomarkers research. Tim Peck (Protasis), Frank Schroeder (Cornell) and Matt Gronquist (SUNY-Fredonia) spoke about CapNMR. Plenary speaker, Jeremy Nicholson (Imperial), was there to explain how ‘Reagentless Diagnostics’ using NMR might improve the quality of human health in the near future. Many health science workers from Roswell Park Cancer Institute added application context. Practical talks continued in the afternoon workshop where Protasis showed a video and set up a live demo of the LEAP HTC liquid handler and One-Minute NMR software. Tim will report on the day's events from his perspective. See all of the action with explanations from Dr. Peck!
Roger Kautz, Northeastern University, Boston, MA Title: Toward An Optimized LC-MS-NMR Strategy: Using a CapNMR Probe With Fully Automated Sample Loading using a Novel Segmented Flow Analysis Loading Technique Some practical techniques for loading and processing routine samples will first be discussed. For LC-MS-NMR analysis, we have integrated two novel LC-MS and NMR technologies to capitalize on the highest sensitivity available for each technique and accommodate the disparities between MS and NMR in their sample mass and time requirements. For LC-MS, conventional 4 mm columns allow validated LC methods and equipment to be used; a NanoSplitter interface provides the phenomenal LC-MS sensitivity of nanoelectrospray, while collecting 100-fold more material for NMR than capillary separations. In an offline NMR strategy the fractions, concentrated by drying in 96-well plates, can be stored until LC-MS data is evaluated and the NMR is available. Comprehensive LC-NMR data of all fractions may be acquired in unattended automation over a night or weekend, with LOD's under 200 ng. In supervised automation or manual loading of single samples, HSQC can be obtained from 20 µg, interpretable 1D spectra from 50 ng, overnight. A "segmented flow" method of handling miniscule samples in microfluidic systems as discrete "plugs", like droplets of water in oil, can load 1 µL samples with negligible (<5%) losses or dilution, increasing sample efficiency several-fold. Robert Albrecht, Director of Automation, Protasis Corporation Title: One-Minute NMR: Precious Samples using HPLC and Capillary NMR, ' Natural Products Analysis The Easy Way with Microplate NMR Automation' Capillary NMR, introduced by Protasis Corporation in 2002 and automated in 2004, is bringing profound changes to High Throughput Natural Products operations in major pharmaceutical companies and research institutions as chemists focus on building libraries of new molecular entities. A driving force for automated purification and structure elucidation of these 'precious' samples, often gathered from remote locations, is the capability of Protasis' One-Minute NMR™ platform to fit into existing LC-MS operations by using laboratory-standard 96- and 384-well microplate consumables, a common well map and compatible data sample lists to rapidly analyze miniscule amounts of sample. Nuclear magnetic resonance augments existing LC-MS operations by providing rich structural information to identify, elucidate, and quantify pure compounds without affecting their chemistry. The Protasis One-Minute NMR system brings non-destructive NMR detection together with analytical HPLC in a way that is practical for quick, routine, front-line analysis. This talk will highlight the ways that the fully-automated system enables the use of single-scan proton NMR experiments to quickly identify and quantify known compounds in HPLC fractions. The use of heteronuclear 2D NMR experiments for full structure elucidation of unknowns will also be discussed. Please register at: http://www.protasis.com/seminar/WhatsNew2007/ Contact David Strand at 508-481-4163 or by email at d.strand@protasis for questions or additional details.